RT PCR Machine

What is RT PCR Machine:

RT-PCR machine is a laboratory instrument used for quantitative analysis of gene expression or detection of RNA and DNA in a biological sample. It is based on the polymerase chain reaction (PCR) technique that amplifies a specific DNA or RNA sequence in the sample to detect and quantify the target sequence. The RT-PCR machine is equipped with a thermal cycler that allows for the amplification of the target sequence in a sample through a series of heating and cooling cycles. The amplified sequence can then be detected and quantified in real-time using fluorescent probes or dyes.

RT-PCR Machine Use:

RT-PCR machines have a wide range of applications in molecular biology and biomedical research. Some of the common uses of RT-PCR machines are:

1. Gene expression analysis: RT-PCR is used to analyze gene expression patterns in cells and tissues. It allows researchers to study how genes are regulated in different biological systems.
2. Pathogen detection: RT-PCR is used to detect and quantify viral and bacterial pathogens in clinical samples such as blood, saliva, and urine. It is a rapid and sensitive method for diagnosing infectious diseases.
3. Genetic testing: RT-PCR is used for genetic testing and screening. It can detect mutations and genetic variations that are associated with inherited disorders.
4. Forensic analysis: RT-PCR is used in forensic analysis to identify individuals based on their DNA profiles. It can also be used to detect and quantify DNA from crime scenes.

Best RT-PCR machine:

When it comes to choosing the best RT-PCR machine, there are several factors to consider, including sensitivity, specificity, throughput, ease of use, and price. Here are some of the top RT-PCR machines on the market:

Thermo Fisher Scientific Applied Biosystems QuantStudio 5 Real-Time PCR System

The Applied Biosystems QuantStudio 5 Real-Time PCR System is a versatile and reliable RT-PCR machine with a range of features, including:

• 96-well and 384-well block formats
• Six different excitation and detection channels
• Up to five-color detection
• Easy-to-use software with automated analysis and reporting capabilities

Bio-Rad CFX96 Touch Real-Time PCR Detection System

The Bio-Rad CFX96 Touch Real-Time PCR Detection System is a popular and user-friendly RT-PCR machine with a range of features, including:

• 96-well and 384-well block formats
• Five different excitation and detection channels
• Up to four-color detection
• Intuitive software with automated analysis and reporting capabilities

Qiagen Rotor-Gene Q MDx Real-Time PCR System

The Qiagen Rotor-Gene Q MDx Real-Time PCR System is a high-throughput RT-PCR machine with a range of features, including:

• 72-well and 96-well block formats
• Five different excitation and detection channels
• Up to four-color detection
• Integrated touch screen and intuitive software with automated analysis and reporting capabilities